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Misonix 4000 water bath sonication unit
4000 Water Bath Sonication Unit, supplied by Misonix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4000+water+bath+sonication+unit/4000+water+bath+sonication+unit/pm38290515-581-28-27
Average 90 stars, based on 1 article reviews
4000 water bath sonication unit - by Bioz Stars, 2026-11
90/100 stars

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Lysis:

Article Title: Compositions and methods for detecting and treating colorectal cancer
Article Snippet: Chromatin Immunoprecipitation (ChIP) ChIP assay was performed according to the protocol (Upstate, Millipore; htt, followed by, p://www.millip, followed by, ore.com/techpublica, followed by, tions/tech1/mcproto407). .. Crosslinking was performed with 1% formaldehyde or 1% paraformaldehyde for 10 min. To enhance cell lysis, we ran the lysate through a 27 g needle three times and flash froze it in −80° C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 min. Protein/DNA complex was precipitated by specific antibodies against H3K79me2, H3K27me3 (Millipore), Stat3 (Cell Signaling) and IgG control (Millipore). .. Then DNA was purified using DNA Purification Kit (Qiagen).

Article Title: Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer.
Article Snippet: First Strand cDNA were reversed with HiScript II 1st Strand cDNA Synthesis Kit (Vazyme, China, Nanjing); and realtime polymerase chain reaction (PCR) were performed with Taq Pro Universal SYBR qPCRMaster Mix (Vazyme, China, Nanjing) according to the procedure. .. To enhance cell lysis, we ran the lysate through a 27g needle three times and flash froze it in 80 C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 20 minutes. .. Protein/DNA complex was precipitated by specific antibodies against H3K4me2/3 (CST) and IgG control (Millipore, Burlington, MA, USA).

Article Title: Compositions and methods for detecting and treating colorectal cancer
Article Snippet: .. To enhance cell lysis, the lysate was run through a 27 g needle three times and flash frozen in −80° C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 minutes. .. Protein/DNA complex was precipitated with specific antibodies against H3K79me2 (abcam, ab3594), STAT3 (Santa Cruz, SC-482) and IgG control (Millipore).

Sonication:

Article Title: Compositions and methods for detecting and treating colorectal cancer
Article Snippet: Chromatin Immunoprecipitation (ChIP) ChIP assay was performed according to the protocol (Upstate, Millipore; htt, followed by, p://www.millip, followed by, ore.com/techpublica, followed by, tions/tech1/mcproto407). .. Crosslinking was performed with 1% formaldehyde or 1% paraformaldehyde for 10 min. To enhance cell lysis, we ran the lysate through a 27 g needle three times and flash froze it in −80° C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 min. Protein/DNA complex was precipitated by specific antibodies against H3K79me2, H3K27me3 (Millipore), Stat3 (Cell Signaling) and IgG control (Millipore). .. Then DNA was purified using DNA Purification Kit (Qiagen).

Article Title: Th22 cells control colon tumorigenesis through STAT3 and Polycomb Repression complex 2 signaling
Article Snippet: .. Sonication was then D ow nl oa de d by [ U ni ve rs ity o f M an ito ba L ib ra ri es ] at 2 2: 44 0 8 Se pt em be r 20 15 performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 min. Protein/DNA complex was precipitated by specific antibodies against H3K27me3 (Millipore), STAT3 (Cell Signaling) and IgG control (Millipore). .. Then DNA was purified using DNA Purification Kit (Qiagen).

Article Title: PRC2 epigenetically silences Th1-type chemokines to suppress effector T cell trafficking in colon cancer
Article Snippet: .. Sonication was performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 minutes. .. Protein/DNA complex was precipitated with specific antibodies against H3K27me3 (6002, Abcam) and IgG control (Millipore).

Article Title: Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer.
Article Snippet: First Strand cDNA were reversed with HiScript II 1st Strand cDNA Synthesis Kit (Vazyme, China, Nanjing); and realtime polymerase chain reaction (PCR) were performed with Taq Pro Universal SYBR qPCRMaster Mix (Vazyme, China, Nanjing) according to the procedure. .. To enhance cell lysis, we ran the lysate through a 27g needle three times and flash froze it in 80 C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 20 minutes. .. Protein/DNA complex was precipitated by specific antibodies against H3K4me2/3 (CST) and IgG control (Millipore, Burlington, MA, USA).

Article Title: DOT1L affects colorectal carcinogenesis via altering T cell subsets and oncogenic pathway
Article Snippet: .. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 minutes. .. Protein/DNA complex was precipitated by specific antibodies against H3K79me2 (Abcam) and IgG control (Millipore).

Article Title: IL-22 + CD4 + T cells promote colorectal cancer stemness via STAT3 transcription factor activation and induction of the methyltransferase DOT1L
Article Snippet: .. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 minutes. .. Western Blot Western blotting was performed with specific antibodies against human STAT3 (9132, Cell Signaling), phosphorylated STAT3 (9138, Cell Signaling), Oct3/4 (sc-5279, Santa Cruz biotechnology), Nanog (ab21624, Abcam), Sox2 (MAB4343, Millipore), H3K79me2 (ab3594, Abcam), H3K9me2 (ab1220, Abcam), H3K9me3 (ab8898, Abcam), H4K20me3 (07-463, Millipore), H3K27me3 (07-449, Millipore), H3K36me3 (ab9050, Abcam), acetyl-Histone H3 (06-599, Millipore), Histone H3 (9715, Cell Signaling), β-Actin (A5441, Sigma), Dot1L (ab72454, Abcam), and Cleaved Notch1 (NICD, ab52301, Abcam).

Article Title: Compositions and methods for detecting and treating colorectal cancer
Article Snippet: .. To enhance cell lysis, the lysate was run through a 27 g needle three times and flash frozen in −80° C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 minutes. .. Protein/DNA complex was precipitated with specific antibodies against H3K79me2 (abcam, ab3594), STAT3 (Santa Cruz, SC-482) and IgG control (Millipore).

Article Title: PRC2 Epigenetically Silences Th1-Type Chemokines to Suppress Effector T-Cell Trafficking in Colon Cancer
Article Snippet: .. Sonication was performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 minutes. .. Protein/DNA complex was precipitated with specific antibodies against H3K27me3 (6002, Abcam) and IgG control (Millipore).

Control:

Article Title: Compositions and methods for detecting and treating colorectal cancer
Article Snippet: Chromatin Immunoprecipitation (ChIP) ChIP assay was performed according to the protocol (Upstate, Millipore; htt, followed by, p://www.millip, followed by, ore.com/techpublica, followed by, tions/tech1/mcproto407). .. Crosslinking was performed with 1% formaldehyde or 1% paraformaldehyde for 10 min. To enhance cell lysis, we ran the lysate through a 27 g needle three times and flash froze it in −80° C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 min. Protein/DNA complex was precipitated by specific antibodies against H3K79me2, H3K27me3 (Millipore), Stat3 (Cell Signaling) and IgG control (Millipore). .. Then DNA was purified using DNA Purification Kit (Qiagen).

Article Title: Th22 cells control colon tumorigenesis through STAT3 and Polycomb Repression complex 2 signaling
Article Snippet: .. Sonication was then D ow nl oa de d by [ U ni ve rs ity o f M an ito ba L ib ra ri es ] at 2 2: 44 0 8 Se pt em be r 20 15 performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 10 min. Protein/DNA complex was precipitated by specific antibodies against H3K27me3 (Millipore), STAT3 (Cell Signaling) and IgG control (Millipore). .. Then DNA was purified using DNA Purification Kit (Qiagen).



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90
Misonix 4000 water bath sonication unit
4000 Water Bath Sonication Unit, supplied by Misonix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4000+water+bath+sonication+unit/4000+water+bath+sonication+unit/pm38290515-581-28-27
Average 90 stars, based on 1 article reviews
4000 water bath sonication unit - by Bioz Stars, 2026-11
90/100 stars
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